Product Pathways - Translational Control
Translation Initiation Complex Antibody Sampler Kit #4763
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| eIF4A (C32B4) Rabbit mAb # 2013 | 40 microliters | W | H M R Mk | 48 | Rabbit |
| eIF4A1 Antibody # 2490 | 40 microliters | W IF-IC | H M R Mk | 48 | Rabbit |
| Phospho-eIF4B (Ser422) Antibody # 3591 | 40 microliters | W | H M R Mk | 80 | Rabbit |
| eIF4B Antibody # 3592 | 40 microliters | W | H M R Mk | 80 | Rabbit |
| Phospho-eIF4E (Ser209) Antibody # 9741 | 40 microliters | W | H M R Mk | 25 | Rabbit |
| eIF4E Antibody # 9742 | 40 microliters | W IHC-P IHC-F | H M R Mk Z | 25 | Rabbit |
| Phospho-eIF4G (Ser1108) Antibody # 2441 | 40 microliters | W IP IF-IC | H M R Mk Hm B | 220 | Rabbit |
| eIF4G (C45A4) Rabbit mAb # 2469 | 40 microliters | W IHC-P IF-IC F | H M R Mk | 220 | Rabbit |
| eIF4H Antibody # 2444 | 40 microliters | W IF-IC | H M R Mk | 25, 27 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
B=Bovine
Z=Zebra Fish
Specificity / Sensitivity
Each antibody in the Translation Initiation Complex Antibody Sampler Kit detects endogenous levels of its target protein.
Western Blotting
Western blot analysis of extracts from various cell lines using eIF4A (C32B4) Rabbit mAb #2013.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody #2441.
Western Blotting
Western blot analysis of extracts from various cell lines using eIF4H Antibody #2444.
Western Blotting
Western blot analysis of extracts from various cell lines using eIF4G (C45A4) Rabbit mAb #2469.
Western Blotting
Western blot analysis of extracts from various cell lines using eIF4A1 Antibody #2490.
Western Blotting
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody #3591 (upper) or eIF4B Antibody #3592 (lower). Forty-eight hours following siRNA transfection cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated.
Western Blotting
Western blot analysis of extracts from NIH/3T3, 293 and HeLa cells using eIF4B Antibody #3592.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody #9741 (upper) or eIF4E Antibody #9742 (lower).
Western Blotting
Western blot analysis of extracts from control and serum treated NIH/3T3, 293 and PC12 cells using eIF4E Antibody #9742.
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Met316 of human eIF4A protein and Gly188 of human eIF4G. Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from a sequence around Gly12 of human eIF4A1, Ser422 of human eIF4B, residues at the amino terminus of human eIF4B, Ser209 of human eIF4E, Ser1108 of human eIF4GI and the sequence of human eIF4H. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7).
- Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608.
- Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-94.
- Sonenberg, N. et al. (1978) Proc Natl Acad Sci U S A 75, 4843-7.
- Gingras, A.C. et al. (1999) Annu Rev Biochem 68, 913-63.
- Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
- Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22.
Application References
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Companion Products
- 2013 eIF4A (C32B4) Rabbit mAb
- 2425 eIF4A (F52) Antibody
- 2490 eIF4A1 Antibody
- 3591 Phospho-eIF4B (Ser422) Antibody
- 3592 eIF4B Antibody
- 9741 Phospho-eIF4E (Ser209) Antibody
- 9742 eIF4E Antibody
- 2441 Phospho-eIF4G (Ser1108) Antibody
- 2469 eIF4G (C45A4) Rabbit mAb
- 2617 eIF4G (C65H5) Rabbit mAb
- 2498 eIF4G Antibody
- 2858 eIF4GI Antibody
- 2444 eIF4H Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
