Product Pathways - Protein Translation
Translation Initiation Complex Antibody Sampler Kit #4763
|4763S||1 Kit (9 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|eIF4A (C32B4) Rabbit mAb #2013||40 µl||W||H, M, R, Mk||48||Rabbit IgG|
|eIF4A1 Antibody #2490||40 µl||W, IF-IC||H, M, R, Mk||48||Rabbit|
|Phospho-eIF4B (Ser422) Antibody #3591||40 µl||W||H, M, R, Mk||80||Rabbit|
|eIF4B Antibody #3592||40 µl||W||H, M, R, Mk||80||Rabbit|
|Phospho-eIF4E (Ser209) Antibody #9741||40 µl||W||H, M, R, Mk||25||Rabbit|
|eIF4E (C46H6) Rabbit mAb #2067||40 µl||W, IP, IHC-P||H, M, R, Mk||25||Rabbit IgG|
|Phospho-eIF4G (Ser1108) Antibody #2441||40 µl||W, IP, IF-IC||H, M, R, Hm, Mk, B||220||Rabbit|
|eIF4G (C45A4) Rabbit mAb #2469||40 µl||W, IHC-P, IF-IC, F||H, M, R, Mk||220||Rabbit IgG|
|eIF4H (D85F2) XP® Rabbit mAb #3469||40 µl||W, IP, IF-IC||H, M, R, Mk||25, 27||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster, B=Bovine
Western blot analysis of extracts from various cell lines using eIF4A (C32B4) Rabbit mAb #2013.
Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb #2067.
Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody #2441.
Western blot analysis of extracts from various cell lines using eIF4G (C45A4) Rabbit mAb #2469.
Western blot analysis of extracts from various cell lines using eIF4A1 Antibody #2490.
Western blot analysis of extracts from HepG2, HeLa and NBT-II cells using eIF4H (D85F2) XP® Rabbit mAb #3469.
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody #3591 (upper) or eIF4B Antibody #3592 (lower). Forty-eight hours following siRNA transfection cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated.
Western blot analysis of extracts from NIH/3T3, 293 and HeLa cells using eIF4B Antibody #3592.
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody #9741 (upper) or eIF4E Antibody #9742 (lower).
The Translation Initiation Complex Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody in the Translation Initiation Complex Antibody Sampler Kit detects endogenous levels of its target protein.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic petide corresponding to residues surrounding Met316 of human eIF4A protein, Gly188 of human eIF4G, and the sequence of human eIF4E and human eIF4H. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Gly12 of human eIF4A1, residues at the amino terminus of human eIF4B, Ser422 of human eIF4B, Ser209 of human eIF4E, and Ser1108 of human eIF4GI. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7).
- Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608.
- Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-94.
- Sonenberg, N. et al. (1978) Proc Natl Acad Sci U S A 75, 4843-7.
- Gingras, A.C. et al. (1999) Annu Rev Biochem 68, 913-63.
- Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
- Rogers, G.W. et al. (2001) J Biol Chem 276, 30914-22.
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- 2013 eIF4A (C32B4) Rabbit mAb
- 2425 eIF4A (F52) Antibody
- 2490 eIF4A1 Antibody
- 3591 Phospho-eIF4B (Ser422) Antibody
- 3592 eIF4B Antibody
- 9741 Phospho-eIF4E (Ser209) Antibody
- 9742 eIF4E Antibody
- 2067 eIF4E (C46H6) Rabbit mAb
- 2441 Phospho-eIF4G (Ser1108) Antibody
- 2469 eIF4G (C45A4) Rabbit mAb
- 2617 eIF4G (C65H5) Rabbit mAb
- 2498 eIF4G Antibody
- 2858 eIF4GI Antibody
- 2444 eIF4H Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.