Product Pathways - NF-kappaB Signaling
NF-κB p65 (C22B4) Rabbit mAb #4764
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk B (Dg) | 65 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
NF-κB p65 (C22B4) Rabbit mAb detects endogenous levels of total NF-κB p65 protein.
Source / Purification
Rabbit monoclonal antibodies were prepared from spleens of rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues near the amino terminus of human NF-κB p65.
Western Blotting
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (C22B4) Rabbit mAb (lower).
Western Blotting
Western blot analysis of extracts from HeLa (human), PC12 (rat) and Neuro2A (mouse) cell lines using NF-κB p65 (C22B4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human melanoma using NF-κB p65 (C22B4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HeLa cells, untreated (left) or treated with TNF-α (right), using NF-κB p65 (C22B4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using NF-κB p65 (C22B4) RmAb #4764 in the presence of control peptide (left) or NF-κB p65 Blocking Peptide #1061 (right).
Flow Cytometry
Flow cytometric analysis of OVCAR8 cells using NF-κB p65 (C22B4) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
Transcription factors of the nuclear factor kappaB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKK-α (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
- Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
- Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
- Haskill, S. et al. (1991) Cell 65, 1281-9.
- Thompson, J.E. et al. (1995) Cell 80, 573-82.
- Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Senftleben, U. et al. (2001) Science 293, 1495-9.
- Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
- Xiao, G. et al. (2001) Mol Cell 7, 401-9.
Application References
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Companion Products
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 3037 Phospho-NF-κB p65 (Ser276) Antibody
- 3039 Phospho-NF-κB p65 (Ser468) Antibody
- 3034 NF-κB p65 Antibody
- 4954 RelB Antibody
- 4727 c-Rel Antibody
- 3035 NF-κB1 p105/p50 Antibody
- 4882 NF-κB2 p100/p52 Antibody
- 4812 IκBα (44D4) Rabbit mAb
- 4814 IκB-α (L35A5) Mouse mAb (Amino-terminal Antigen)
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
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- 1061 NF-κB p65 Blocking Peptide
- 9243 NF-κB Control Cell Extracts
- 9936 NF-κB Pathway Sampler Kit
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
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- 7003 20X LumiGLO® Reagent and 20X Peroxide