Product Pathways - Apoptosis
Tid-1 (RS13) Mouse mAb #4775
|W IP||H M R||Endogenous||37 Tid-1s. 40 Tid-1L.||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Tid-1 (RS13) Mouse Monoclonal Antibody detects endogenous levels of the short and long variants of Tid-1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human Tid-1 protein. Antibody is supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 mg/ml BSA and 50% glycerol.
Human Tid-1 is a human orthologue of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l (2) tid and is a member of the DnaJ family of proteins that serve as co-chaperones to Hsp70 proteins (1). These proteins are characterized by a J domain, a highly conserved tetrahelical domain that binds to Hsp70 chaperones and activates their ATPase activity. Hsp70 and their associated chaperones mediate a variety of activities including the folding of newly synthesized polypeptides, the translocation of proteins across membranes and assembly of multimeric protein complexes. Two alternatively spliced variants exist for human Tid-1 ,designated hTID-1s and hTID-1L, both which contain the J domain, localize to the mitochondrial matrix, and co-immunoprecipitate with Hsp70. Expression of Tid-1L increases apoptosis induced by the DNA damaging agent mitomycin c (MMC) and by TNF-alpha, and that activity is dependent on its J domain. In contrast, expression of Tid-1S reduces apoptosis by these agents. Tid-1 orthologues are also found in mouse (mTid-1) and rat (rTid-1) (2,3). The mouse orthologue was originally identified though its interaction with p120 GTPase-activating protein (GAP), raising the possiblity that Tid-1 helps regulates the confirmation, activity, or subcellular localization of GAP (3).
- Syken, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8499-8504.
- Fujita, M. et al. (2004) Mol. Cell Biochem. 258, 183-189.
- Trentin, G. A. et al. (2001) J. Biol. Chem. 276, 13087-13095.
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For Research Use Only. Not For Use In Diagnostic Procedures.