Cell Signaling Technology

Product Pathways - Translational Control

hnRNP LL Antibody #4783

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 61 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

hnRNP LL Antibody detects endogenous levels of total hnRNP LL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human hnRNP LL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 and HeLa cells using hnRNP LL Antibody.

Background

Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL) is a nuclear RNA-binding protein that shares 69% amino acid homology with hnRNP L, a component of the hnRNP complex that regulates mRNA formation, packaging, and processing (1). hnRNP LL is induced in activated T cells and functions as a critical regulator of alternative splicing of CD45, a tyrosine phosphatase crucial for T cell development and activation (2). hnRNP LL also regulates the splicing of CD44 and Stat5a (2). The four isoforms of hnRNP LL are generated by alternative splicing and are widely expressed in human tissues (3).

  1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
  2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
  3. Shur, I. et al. (2004) Gene 334, 113-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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