Cell Signaling Technology

Product Pathways - Translational Control

hnRNPLL Antibody #4783

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 61 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

hnRNPLL Antibody detects endogenous levels of total hnRNPLL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human hnRNPLL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 and HeLa cells using hnRNPLL Antibody.

Background

Heterogeneous nuclear ribonucleoprotein L-like (hnRNPLL) is a nuclear RNA-binding protein that shares 69% amino acid homology with hnRNPL, a component of hnRNP complex which regulates mRNA formation, packaging, and processing (1). hnRNPLL is induced in activated T-cells and functions as a critical regulator of alternative splicing of CD45, a tyrosine phosphatase crucial for T-cell development and activation (2). hnRNPLL also regulates the splicing of CD44 and STAT5A (2). Four isoforms of hnRNPLL, generated by alternative splicing, are widely expressed in human tissues (3).

  1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
  2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
  3. Shur, I. et al. (2004) Gene 334, 113-21.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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