Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Protein Stability

Ubc9 (D26F2) XP® Rabbit mAb #4786

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Endogenous 17 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ubc9 (D26F) XP® Rabbit mAb detects endogenous levels of total Ubc9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile125 of human Ubc9 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ubc9 (D26F2) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A172 cells using Ubc9 (D26F2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification (1). Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3 (2). Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme (3). Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain (3,4). Localization of Ubc9 to the nucleus and the nuclear envelope allows this enzyme to catalyze target protein sumoylation and regulate target protein nucleocytoplasmic transport and transcriptional activity (5,6). Ubc9 target proteins include a host of proteins (RAD51, RAD52, p53 and c-Jun) that regulate the cell cycle, DNA repair, and p53-dependent processes (7).

  1. Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56.
  2. Tatham, M.H. et al. (2003) Biochemistry 42, 3168-79.
  3. Sampson, D.A. et al. (2001) J Biol Chem 276, 21664-9.
  4. Liu, Q. et al. (1999) J Biol Chem 274, 16979-87.
  5. Lee, G.W. et al. (1998) J Biol Chem 273, 6503-7.
  6. Pichler, A. and Melchior, F. (2002) Traffic 3, 381-7.
  7. Mo, Y.Y. and Moschos, S.J. (2005) Expert Opin Ther Targets 9, 1203-16.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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