Product Pathways - Apoptosis
Caspase-8 (D35G2) Rabbit mAb #4790
PhosphoSitePlus® protein, site, and accession data: Casp8
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R (Mk) (Pg) | Endogenous | 10, 57 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4790:
- Western Blotting
Specificity / Sensitivity
Caspase-8 (D35G2) Rabbit mAb detects endogenous levels of total caspase-8, including the p10 subunit of the activated protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human caspase-8 protein.
Western Blotting
Western blot analysis of extracts from HeLa, THP-1, and YB2/0 cell lines using Caspase-8 (D35G2) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 4 hours), using Caspase-8 (D35G2) Rabbit mAb.
Background
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
- Muzio, M. et al. (1996) Cell 85, 817-827.
- Boldin, M.P. et al. (1996) Cell 85, 803-815.
- Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.
Application References
- Pritchard, J.R. et al. (2011) Cancer Res 71, 5850-8. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
