Product Pathways - Tyrosine Kinase/ Adaptors
HER4/ErbB4 (83B10) Rabbit mAb (IHC Specific) #4792
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H | Transfected Only | 180 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 4792:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
HER4/ErbB4 (111B2) Rabbit mAb detects transfected levels of ErbB4 protein. This antibody does not cross-react with other ErbB family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ErbB4.
Western Blotting
Western blot analysis of cell extracts from various cell lines, using HER4/ErbB4 (83B10) Rabbit mAb (IHC Specific). This antibody specifically detects overexpressed HER4/ErbB4 proteins in tranfected Cos cells, but does not cross-react with EGFR (A431 and MDA-468), ErbB2 (MDA-453) and ErbB3 (MCF7 and T47D) proteins.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using HER4/ErbB4 (83B10) Rabbit mAb (IHC Specific) #4792 in the presence of control peptide (left) or HER4/ErbB4 Blocking Peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HER4/ErbB4 (83B10) Rabbit mAb (IHC Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus, showing cytoplasmic localization, using HER4/ErbB4 (83B10) Rabbit mAb (IHC Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HER4/ErbB4 (83B10) Rabbit mAb (IHC Specific).
Background
The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).
- Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
- Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
- Carpenter, G. (2003) Exp Cell Res 284, 66-77.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 1022 HER4/ErbB4 Blocking Peptide
- 4757 Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb
- 4795 HER4/ErbB4 (111B2) Rabbit mAb
- 3790 Phospho-HER4/ErbB4 (Tyr984) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
