Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RalA (D6G8) Rabbit mAb #4799

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 25 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RalA (D6G8) Rabbit mAb detects endogenous levels of total RalA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala142 of human RalA protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A172, COS-7 and Neuro-2A cells using RalA (D6G8) Rabbit mAb.

Background

RalA and RalB are members of the Ras family of small GTPases and are highly homologous in protein sequence. The functions of RalA and RalB are distinct yet overlapping. By binding to various effector proteins, RalA and RalB serve as important GTP sensors for exocytosis and membrane trafficking (1-3). RalA is required for Ras-related tumorigenesis (4) and RalB is important for tumor survival (5). In addition to tumor formation, Ral proteins also play a role in cancer cell migration and metastatic tumor invasion (6,7).

  1. Li, G. et al. (2007) J Neurosci 27, 190-202.
  2. van Dam, E.M. and Robinson, P.J. (2006) Int J Biochem Cell Biol 38, 1841-7.
  3. Camonis, J.H. and White, M.A. (2005) Trends Cell Biol 15, 327-32.
  4. Lim, K.H. et al. (2005) Cancer Cell 7, 533-45.
  5. Chien, Y. et al. (2006) Cell 127, 157-70.
  6. Oxford, G. et al. (2005) Cancer Res 65, 7111-20.
  7. Lim, K.H. et al. (2006) Curr Biol 16, 2385-94.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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