Cell Signaling Technology

Product Pathways - NF-kB Signaling

RAGE 1 Antibody #4800

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H (Mk) Endogenous 58 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RAGE 1 Antibody detects endogenous levels of total RAGE 1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RAGE isoform 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extract from human lung tissue using RAGE 1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human normal lung using Rage 1 Antibody.

Background

The receptor for advanced glycation end products (RAGE) is member of the immunoglobulin (Ig) superfamily. It can be expressed as full-length, membrane-bound RAGE isoform 1 or as a secreted sRAGE protein that lacks a transmembrane domain (1). RAGE is detected during early developmental stages and in the lung under normal physiological conditions (2) and is upregulated at sites of inflammation (3). Advanced glycation end products (AGEs) and a variety of other ligands interact with this receptor (1). Ligand binding activates full-length RAGE and initiates downstream signaling pathways that include activation of NF-κB, which leads to production of pro-inflammatory cytokines and inflammation (4). Activation of these pathways has been implicated in various disease states including Alzheimer disease, diabetes, arthritis, and atherosclerosis (4). Soluble RAGE can competitively bind RAGE ligands in the extracellular environment, which prevents ligand interaction with full-length RAGE at the cell surface (1).

  1. Bierhaus, A. et al. (2005) J Mol Med 83, 876-86.
  2. Brett, J. et al. (1993) Am J Pathol 143, 1699-712.
  3. Sparvero, L.J. et al. (2009) J Transl Med 7, 17.
  4. Lin, L. et al. (2009) Front Biosci 14, 1403-13.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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