Product Pathways - DNA Damage
SMC1 Antibody #4802
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M (R) (C) (B) (X) | Endogenous | 145 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
B=Bovine
X=Xenopus
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
SMC1 Antibody detects endogenous levels of total SMC1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with synthetic peptides (KLH-coupled) corresponding to residues near the amino terminus of human SMC1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 cells, untreated or UV-treated, using Phospho-SMC1 (Ser957) Antibody #4801 (upper) or SMC1 Antibody (lower).
Flow Cytometry
Flow cytometric analysis of HeLa cells, using SMC1 antibody (blue) compared to a nonspecific negative control antibody (red).
Background
Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). In response to ionizing radiation (IR), as part of the intra-S-phase DNA damage checkpoint, ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 (3). In cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment, SMC1 phosphorylation is ATM-independent (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).
- Michaelis, C. et al. (1997) Cell 91, 35-45.
- Sjogren, C. and Nasmyth, K. (2001) Curr. Biol. 11, 991-995.
- Yazdi, P.T. et al. (2002) Genes Dev. 16, 571-582.
- Kim, S.T. et al. (2002) Genes Dev. 16, 560-570.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4801 Phospho-SMC1 (Ser957) Antibody
- 4805 Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb
- 4526 Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb
- 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
- 2577 Phospho-Histone H2A.X (Ser139) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
