Product Pathways - Translational Control
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4803
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H (M) (R) (Mk) | Endogenous | Rabbit IgG |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of S6 ribosomal protein only when phosphorylated at Ser235 and Ser236.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human S6 ribosomal protein. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002/wortmannin/U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 488 Conjugate).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, treated with wortmannin and U0126 (left) or PMA (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 488 Conjugate). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody, #4858, reacts with human, mouse, rat and monkey Phospho-S6 Ribosomal protein. CST expects that Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize phospho-S6 ribosomal protein in these species.
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
- 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 4851 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 2215 Phospho-S6 Ribosomal Protein (Ser240/244) Antibody
- 4838 Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb
- 2317 S6 Ribosomal Protein (54D2) Mouse mAb
- 4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
