Product Pathways - DNA Damage
Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M (B) | Endogenous | 145 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957. This antibody may also recognize phosphorylated human DNA-PKcs (450kDa).
Source / Purification
Monoclonal antibodies are produced by immunizing mice with a synthetic phospho-peptide corresponding to residues surrounding Ser957 of human SMC1.
Western Blotting
Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody (upper), SMC1 Antibody #4802 (middle), and DNA-PK Antibody #4602 (lower).
Background
Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). In response to ionizing radiation (IR), as part of the intra-S-phase DNA damage checkpoint, ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 (3). In cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment, SMC1 phosphorylation is ATM-independent (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).
- Michaelis, C. et al. (1997) Cell 91, 35-45.
- Sjogren, C. and Nasmyth, K. (2001) Curr. Biol. 11, 991-995.
- Yazdi, P.T. et al. (2002) Genes Dev. 16, 571-582.
- Kim, S.T. et al. (2002) Genes Dev. 16, 560-570.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4801 Phospho-SMC1 (Ser957) Antibody
- 4802 SMC1 Antibody
- 4526 Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb
- 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
- 4602 DNA-PK Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
