Product Pathways - DNA Damage
Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805
PhosphoSitePlus® protein, site, and accession data: Smc1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M (B) | Endogenous | 145 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4805:
- Western Blotting
Specificity / Sensitivity
Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957. This antibody may also recognize phosphorylated human DNA-PKcs (450kDa).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1.
Western Blotting
Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody (upper), SMC1 Antibody #4802 (middle), and DNA-PK Antibody #4602 (lower).
Background
Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).
- Michaelis, C. et al. (1997) Cell 91, 35-45.
- Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
- Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
- Kim, S.T. et al. (2002) Genes Dev 16, 560-70.
Application References
- Biton, S. and Ashkenazi, A. (2011) Cell 145, 92-103. Applications: Western Blotting
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Companion Products
- 4801 Phospho-SMC1 (Ser957) Antibody
- 4802 SMC1 Antibody
- 4526 Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb
- 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
- 4602 DNA-PK Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
