Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat5a (4H1) Mouse mAb #4807

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP F H Endogenous 90 Mouse IgG2b

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Stat5a (4H1) Mouse mAb detects endogenous levels of total human Stat5a protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment expressing amino acids 2-104 of human Stat5a.

Western Blotting

Western Blotting

Western blot analyis of extracts from K-562, MOLT-4 and TF-1 cells using Stat5a (4H1) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (red) and K-562 cells (blue) using Stat5a (4H1) Mouse mAb.

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

  1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369.
  2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.
  4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.
  5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
  6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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