Product Pathways - NF-kB Signaling

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Phospho-NF-κB p105 (Ser933) (178F3) Rabbit mAb (IHC Specific) #4808

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
IHC-P	H	Endogenous	105	Rabbit IgG

Applications Key:  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4808:

IHC / Paraffin

Specificity / Sensitivity

Phospho-NF-kappaB p105 (Ser933) (178F3) Rabbit mAb detects endogenous levels of p105NF-kappaB only when phosphorylated at serine 933.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Ser933 of NF-kappaB p105.

Background

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

Following IKK-mediated phosphorylation of p105 NF-kappaB at multiple sites (Ser921, 923, 927 and 933) on its carboxy-terminus, SCFbeta-TrCP mediated processing produces the 50 kDa active form p50 (12,13).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.
12. Heissmeyer, V. et al. (2001) Mol. Cell. Biol. 21, 1024-1035.
13. Orian, A. et al. (2000) EMBO J. 19, 2580-2591.

Application References

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Companion Products

• 4806 Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb
• 1021 Phospho-NF-kappaB p105 (Ser933) Blocking Peptide
• 3035 NF-κB1 p105/p50 Antibody
• 4764 NF-κB p65 (C22B4) Rabbit mAb
• 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
• 9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.