Product Pathways - NF-kB Signaling

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

IκBα (44D4) Rabbit mAb #4812

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H M R Hm Mk Mi	Endogenous	39	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Mi=Mink
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4812:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

IκBα (44D4) Rabbit mAb detects endogenous levels of total IκBα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human IκBα.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

• Dai, P. et al. (2009) J Immunol 182, 3450-60. Applications: Western Blotting
• Xie, S. et al. (2010) J Immunol 184, 2289-96. Applications: Western Blotting
• Palkowitsch, L. et al. (2011) J Biol Chem 286, 7522-34. Applications: Western Blotting
• Tokunaga, F. et al. (2012) EMBO J 31, 3856-70. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
• 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
• 9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb
• 4764 NF-κB p65 (C22B4) Rabbit mAb
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 2682 IKKα Antibody
• 2370 IKKβ (2C8) Rabbit mAb
• 2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
• 9936 NF-κB Pathway Sampler Kit
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

---

For Research Use Only. Not For Use In Diagnostic Procedures.