Cell Signaling Technology

Product Pathways - Neuroscience

GABA(B)R2 (C44A4) Rabbit mAb #4819

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 105 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GABA(B)R2 (C44A4) Rabbit mAb detects endogenous levels of total GABA(B)R2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln430 of human GABA(B)R2.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brains using GABA(B)R2 (C44A4) Rabbit mAb.

Background

GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

  1. Jones, K.A. et al. (2000) Neuropsychopharmacology 23, S41-9.
  2. Duthey, B. et al. (2002) J Biol Chem 277, 3236-41.
  3. Kaupmann, K. et al. (1997) Nature 386, 239-46.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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