Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calmodulin Antibody #4830

Applications Reactivity Sensitivity MW (kDa) Source
W M R Endogenous 17 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Calmodulin Antibody detects endogenous levels of total calmodulin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Glu140 of human calmodulin. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat and mouse brain using Calmodulin Antibody.

Background

Calmodulin is a ubiquitously expressed small protein mediating many cellular effects such as short-term and long-term memory, nerve growth, inflammation, apoptosis, muscle contraction and intracellular movement (1). Upon binding of four Ca2+ ions, calmodulin undergoes conformational changes, allowing this complex to bind to and activate many enzymes including protein kinases, protein phosphatases, ion channels, Ca2+ pumps, nitric oxide synthase, inositol triphosphate kinase, and cyclic nucleotide phosphodiesterase (2,3). Since calmodulin binds Ca2+ in a cooperative fashion, small changes in cytosolic Ca2+ levels lead to large changes in the level of active calmodulin and its target proteins (4).

  1. Persechini, A. et al. (1989) Trends Neurosci 12, 462-7.
  2. Finn, B.E. et al. (1995) Nat Struct Biol 2, 777-83.
  3. Agell, N. et al. (2002) Cell Signal 14, 649-54.
  4. Porter, J.A. et al. (1993) Science 262, 1038-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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