Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Protein Stability

HAUSP (D17C6) XP® Rabbit mAb #4833

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 135, 140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HAUSP (D17C6) XP® Rabbit mAb detects endogenous levels of total HAUSP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human HAUSP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HAUSP (D17C6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using HAUSP (D17C6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function is to bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins (3,4). In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR (5,6).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Li, M. et al. (2002) Nature 416, 648-53.
  4. Brooks, C.L. et al. (2007) Oncogene 26, 7262-6.
  5. van der Horst, A. et al. (2006) Nat Cell Biol 8, 1064-73.
  6. Oh, Y.M. et al. (2007) Biochem Biophys Res Commun 357, 615-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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