Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

MARK4 Antibody #4834

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M R Endogenous 79 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

MARK4 Antibody detects endogenous levels of MARK4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence surrounding Cys514 of human MARK4. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji (human), C2C12 (mouse), and NBT-II (rat) cells, using MARK4 Antibody.

IF-IC

IF-IC

Immunofluorescent analysis of C2C12 cells, using MARK4 Antibody

Background

Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).

  1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-344.
  2. Illenberger, S. et al. (1996) J. Biol. Chem. 271, 10834-10843.
  3. Drewes, G. et al. (1995) J. Biol. Chem. 270, 7679-7688.
  4. Drewes, G. et al. (1997) Cell 89, 297-308.
  5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
  6. Trinczek, B. et al. (2004) J. Biol. Chem. 279, 5915-5923.
  7. Lizcano, J. M. et al. (2004) EMBO J. 23, 833-843.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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