Cell Signaling Technology

Product Pathways - NF-kB Signaling

Cox1 Antibody #4841

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H M R Mk Endogenous 65, 70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Cox1 Antibody Antibody detects endogenous levels of total Cox1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of mouse Cox1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 and Neuro2A cells using Cox1 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells using Cox1 Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). Cox1 is expressed constitutively in most tissues, while Cox2 expression is induced by lipopolysaccaride (LPS) and peptoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk 1/2 at Tyr204). The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκB-α at Ser32/36) and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever and septic shock (5), making Cox2 a useful target for therapeutic anti-inflammatory drugs (3). Both Cox1 and Cox2 also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).

  1. Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
  2. Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
  3. O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
  4. Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
  5. Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
  6. Qin, W. et al. (2003) J Biol Chem 278, 50970-7.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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