Cell Signaling Technology

Product Pathways - NF-kB Signaling

Cox1 Antibody #4841

Applications Reactivity Sensitivity MW (kDa) Source
W F H M R Endogenous 65, 70 Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cox1 Antibody Antibody detects endogenous levels of total Cox1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of mouse Cox1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 and Neuro2A cells using Cox1 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells using Cox1 Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).

  1. Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
  2. Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
  3. O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
  4. Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
  5. Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
  6. Qin, W. et al. (2003) J Biol Chem 278, 50970-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products