Product Pathways - NF-kB Signaling
Cox2 Antibody #4842
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PhosphoSitePlus® protein, site, and accession data: COX-2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M (R) | Endogenous | 74 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4842:
- Western Blotting
Specificity / Sensitivity
Cox2 Antibody detects endogenous levels of total Cox2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Cox2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot of extracts from Raw264.7 cells, untreated or LPS-treated (1 µg/ml for 6 h), using Cox2 Antibody.
Western Blotting
Western blot analysis of extracts from Raw264.7 cells, untreated or LPS-treated (1 μg/ml for 6 h), using Cox2 Antibody.
Background
Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).
- Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
- Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
- O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
- Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
- Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
- Qin, W. et al. (2003) J Biol Chem 278, 50970-7.
Application References
- Lee, J.K. et al. (2004) Proc Natl Acad Sci U S A 101, 8815-20. Applications: Western Blotting
- Forrester, M.T. et al. (2011) Biotechniques 50, 41-5. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
