Product Pathways -
Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) #4843
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | Endogenous | Mouse IgG1 |
Applications Key:
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4843:
- Flow
Specificity / Sensitivity
The antibody is not directed against any known antigen. It functions as an isotype control for mouse monoclonal antibodies. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6.
Source / Purification
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Akt (5G3) Mouse mAb (Alexa Fluor® 647 Conjugate) #2944 (green).
Flow Cytometry
Flow cytometric analysis of U0126-treated Jurkat cells using Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) (red) compared to Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (Alexa Fluor® 647 Conjugate) #4375, U0126-treated (blue) or PMA-treated (green).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis of human cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
- Nishimoto, N. et al. (2011) Blood 118, 2541-50. Applications: Flow Cytometry
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
