Product Pathways - Apoptosis / Autophagy
COX IV Antibody #4844
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk B | Endogenous | 17 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
COX IV Antibody detects endogenous levels of total COX IV protein.
Source / Purification
Polyclonal antibodies were prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa (human), C6 (rat) and NIH/3T3 (mouse) cell lines using COX IV Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human adenoma of the large intestine, using COX IV Antibody in the presence of control peptide (left) or antigen-specific peptide #1034 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic localization using COX IV Antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using Cox IV Antibody (blue) compared to a nonspecifc negative control antibody (red).
IF-IC
Immunofluorescence staining of HeLa cells, showing mitochondrial staining, using COX IV Antibody.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Rad. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
- Endo, H. et al. (2006) Biochem Soc Trans 34, 1283-6. This article references the use of COX IV Antibody in the following applications: IF-IC (In Cells)
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4967 β-Actin Antibody
- 4272 Cytochrome c Antibody
- 4850 COX IV (3E11) Rabbit mAb
- 4853 COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 1034 COX IV Blocking Peptide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
