Cell Signaling Technology

Product Pathways - Apoptosis

COX IV Antibody #4844

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P F H M R Mk B Endogenous 17 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

COX IV Antibody detects endogenous levels of total COX IV protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa (human), C6 (rat) and NIH/3T3 (mouse) cell lines using COX IV Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human adenoma of the large intestine, using COX IV Antibody in the presence of control peptide (left) or antigen-specific peptide #1034 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic localization using COX IV Antibody.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using Cox IV Antibody (blue) compared to a nonspecifc negative control antibody (red).

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

  1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
  3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
  4. Barrientos, A. et al. (2002) Gene 286, 53-63.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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