Product Pathways - Apoptosis
COX IV Antibody #4844
|W IP IHC-P F||H M R Mk B||Endogenous||17||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
COX IV Antibody detects endogenous levels of total COX IV protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa (human), C6 (rat) and NIH/3T3 (mouse) cell lines using COX IV Antibody.
Immunohistochemical analysis of paraffin-embedded human adenoma of the large intestine, using COX IV Antibody in the presence of control peptide (left) or antigen-specific peptide #1034 (right).
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic localization using COX IV Antibody.
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
- Csiszar, A. et al. (2007) Am J Physiol Heart Circ Physiol 293, H919-27. Applications: Western Blotting
- Steckley, D. et al. (2007) J Neurosci 27, 12989-99. Applications: Western Blotting
- Li, B. et al. (2008) Endocrinology , . Applications: Western Blotting
- Ungvari, Z. et al. (2008) Am J Physiol Heart Circ Physiol 294, H2121-8. Applications: Western Blotting
- Ye, B. et al. (2009) Circ Res 105, 1083-93. Applications: Western Blotting
- Panduri, V. et al. (2006) Am J Respir Cell Mol Biol 34, 443-52. Applications: Western Blotting
- Widenmaier, S.B. et al. (2009) J Biol Chem 284, 30372-82. Applications: Western Blotting
- Zhao, L. et al. (2012) J Biol Chem 287, 1054-65. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.