Cell Signaling Technology

Product Pathways - NF-kB Signaling

RANK Antibody #4845

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RANK Antibody detects endogenous levels of RANK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of RANK. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 (human), KNRK (rat) and C2C12 (mouse) cell lines, using RANK Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells mock transfected or transfected with mRANK, using RANK Antibody.

Background

RANK (receptor activator of NF-κB) is a member of the tumor necrosis factor (TNF) receptor subfamily that is activated by its ligand, RANKL (TRANCE/OPGL/ODF), to promote survival of dendritic cells and differentiation of osteoclasts (1-4). Although RANK is widely expressed, its cell surface expression may be more restricted to dendritic cells and foreskin fibroblasts (1). RANK contains a 383-amino acid intracellular domain that associates with specific members of the TRAF family to NF-κB and JNK activiation (1,5). RANKL/RANK signaling may also lead to survival signaling through activation of the Akt pathway and an upregulation of survival proteins, including Bcl-xL (2,6). RANK signaling has been implicated as a potential therapeutic to inhibit bone loss and arthritis (7,8).

  1. Anderson, D.M. et al. (1997) Nature 390, 175-9.
  2. Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
  3. Lacey, D.L. et al. (1998) Cell 93, 165-76.
  4. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
  5. Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
  6. Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
  7. Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
  8. Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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