Cell Signaling Technology

Product Pathways - Adhesion

DOCK180 (C4C12) Rabbit mAb #4846

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 215 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DOCK180 (C4C12) Rabbit mAb recognizes endogenous levels of total DOCK180 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human DOCK180.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types, using DOCK180 (C4C12) Rabbit mAb.

IP

IP

Immunoprecipitation of DOCK180 from HCT15 cells, using DOCK180 (C4C12) Rabbit mAb. Western blot was performed using the same antibody.

Background

DOCK180 and its partner, ELMO1, interact directly with one another to form an atypical two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). Rac activation occurs in association with p130 Cas and Crk, which form a complex with DOCK180 that is targeted to focal adhesions (2,3). DOCK180 is also recruited to the plasma membrane by binding to phosphoinositides (4). ELMO1 may function as an inhibitor of proteasome-dependent degradation of DOCK180 at the plasma membrane to regulate reorganization of the actin cytoskeleton (5). Localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes such as phagocytosis and cell migration (6-8).

  1. Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
  2. Matsuda, M. et al. (1996) J. Biol. Chem. 271, 14468-14472.
  3. Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
  4. Côté, J.F. et al. (2005) Nat. Cell Biol. 7, 797-807.
  5. Makino, Y. et al. (2006) J. Cell Sci. 119, 923-932.
  6. Takenawa, T. and Miki, H. (2001) J. Cell Sci. 114, 1801-1809.
  7. Albert, M.L. et al. (2000) Nat. Cell Biol. 2, 899-905.
  8. Gustavsson, A. et al. (2004) J. Biol. Chem. 279, 22893-22901.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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