Product Pathways - Adhesion
DOCK180 (C4C12) Rabbit mAb #4846
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 215 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
DOCK180 (C4C12) Rabbit mAb recognizes endogenous levels of total DOCK180 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal sequence of human DOCK180.
Western Blotting
Western blot analysis of extracts from various cell types, using DOCK180 (C4C12) Rabbit mAb.
IP
Immunoprecipitation of DOCK180 from HCT15 cells, using DOCK180 (C4C12) Rabbit mAb. Western blot was performed using the same antibody.
Background
DOCK180 and its partner, ELMO1, interact directly with one another to form an atypical two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). Rac activation occurs in association with p130 Cas and Crk, which form a complex with DOCK180 that is targeted to focal adhesions (2,3). DOCK180 is also recruited to the plasma membrane by binding to phosphoinositides (4). ELMO1 may function as an inhibitor of proteasome-dependent degradation of DOCK180 at the plasma membrane to regulate reorganization of the actin cytoskeleton (5). Localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes such as phagocytosis and cell migration (6-8).
- Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
- Matsuda, M. et al. (1996) J. Biol. Chem. 271, 14468-14472.
- Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
- Cote, J.F. et al. (2005) Nat. Cell Biol. 7, 797-807.
- Makino, Y. et al. (2006) J. Cell Sci. 119, 923-932.
- Takenawa, T. and Miki, H. (2001) J. Cell Sci. 114, 1801-1809.
- Albert, M.L. et al. (2000) Nat. Cell Biol. 2, 899-905.
- Gustavsson, A. et al. (2004) J. Biol. Chem. 279, 22893-22901.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
