Product Pathways - Adhesion
DOCK180 (C4C12) Rabbit mAb #4846
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 215 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
DOCK180 (C4C12) Rabbit mAb recognizes endogenous levels of total DOCK180 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal sequence of human DOCK180.
Background
DOCK180 and its partner, ELMO1, interact directly with one another to form an atypical two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). Rac activation occurs in association with p130 Cas and Crk, which form a complex with DOCK180 that is targeted to focal adhesions (2,3). DOCK180 is also recruited to the plasma membrane by binding to phosphoinositides (4). ELMO1 may function as an inhibitor of proteasome-dependent degradation of DOCK180 at the plasma membrane to regulate reorganization of the actin cytoskeleton (5). Localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes such as phagocytosis and cell migration (6-8).
- Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
- Matsuda, M. et al. (1996) J. Biol. Chem. 271, 14468-14472.
- Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
- Cote, J.F. et al. (2005) Nat. Cell Biol. 7, 797-807.
- Makino, Y. et al. (2006) J. Cell Sci. 119, 923-932.
- Takenawa, T. and Miki, H. (2001) J. Cell Sci. 114, 1801-1809.
- Albert, M.L. et al. (2000) Nat. Cell Biol. 2, 899-905.
- Gustavsson, A. et al. (2004) J. Biol. Chem. 279, 22893-22901.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.