Product Pathways - Cytoskeletal Signaling
N-WASP (30D10) Rabbit mAb #4848
|4848S||100 µl (10 western blots)||---||In Stock||---|
|4848||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Bovine||Endogenous||65||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
N-WASP (30D10) Rabbit mAb recognizes endogenous levels of total N-WASP protein. The antibody does not cross-react wth the hematopietic protein, WASP.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding the sequence of human N-WASP.
Western blot analysis of extracts from various cell types, using N-WASP (30D10) Rabbit mAb.
Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).
- Millard, T.H. et al. (2004) Biochem J. 380, 1-17.
- Yan, C. et al. (2003) EMBO J. 22, 3602-3612.
- Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904.
- Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132.
- Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302.
- Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974.
- Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145.
- Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 4860 WASP Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.