Product Pathways - Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb #4850
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F | H R Mk B | Endogenous | 17 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb detects endogenous levels of total COX IV protein.
Source / Purification
Rabbit monoclonal antibodies were prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, using Cox IV (3E11) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Rad. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4844 COX IV Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4967 β-Actin Antibody
- 4272 Cytochrome c Antibody
- 1034 COX IV Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
