Product Pathways - Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb #4850
|W IP IHC-P IHC-F IF-IC F||H R Mk Z B Pg||Endogenous||17||Rabbit IgG|
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).
Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.
Immunohistochemical analysis of frozen H1650 xenograft, using Cox IV (3E11) Rabbit mAb.
Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
- Aschrafi, A. et al. (2008) J Neurosci 28, 12581-90. Applications: Western Blotting
- Obexer, P. et al. (2009) J Biol Chem 284, 30933-40. Applications: Western Blotting
- Nagata, N. et al. (2012) Endocrinology 153, 3158-69. Applications: Western Blotting
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4967 β-Actin Antibody
- 4272 Cytochrome c Antibody
- 1034 COX IV Blocking Peptide
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 8112 SignalStain® Antibody Diluent
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.