Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853

Applications Reactivity Sensitivity Isotype
IF-F IF-IC F H R Mk Z B Pg Endogenous Rabbit IgG

Applications Key:  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  R=Rat  Mk=Monkey  Z=Zebrafish  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total COX IV protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 (blue) and Jurkat cells (green) using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SKOV3 cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

IF-F

IF-F

Confocal immunofluorescent analysis of frozen HCC827 xenograft using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Note specific staining of human cancer cells. Blue pseudocolor = DRAQ® #4084 (fluorescent DNA dye).


Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

  1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
  3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
  4. Barrientos, A. et al. (2002) Gene 286, 53-63.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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