Product Pathways - Cytoskeletal Signaling

COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853

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Applications	Reactivity	Sensitivity	Isotype
IF-F IF-IC F	H R Mk	Endogenous	Rabbit IgG

Applications Key:  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total COX IV protein. The unconjugated antibody #4850 reacts with human, rat and monkey COX IV. CST expects that COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize COX IV in these species.

Source / Purification

Rabbit monoclonal antibodies were prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-5.

Description

This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control.

1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
3. Kadenbach, B. et al. (2000) Free Rad. Biol. Med. 29, 211-221.
4. Barrientos, A. et al. (2002) Gene 286, 53-63.

Application References

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Companion Products

• 4967 β-Actin Antibody
• 4272 Cytochrome c Antibody
• 2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor^® 555 Conjugate)

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.