Product Pathways - Translational Control
Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IF-IC F | H M R | 32 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 2215 Phospho-S6 Ribosomal Protein (Ser240/244) Antibody
- 2217 S6 Ribosomal Protein (5G10) Rabbit mAb
- 1155 S6 Ribosomal Protein Blocking Peptide
- 4857 Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb (IHC Preferred)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
