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Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857

Have you tried your application using our XP^® monoclonal antibodies? Try product: 4858

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IHC-F IF-IC	H M R	Endogenous	32	Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4857:

IHC / Frozen, IHC / Paraffin, Immunofluorescence, Western Blotting

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 235 and 236.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

1. Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
3. Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
4. Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
5. Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.

Application References

• Benedettini, E. et al. (2010) Am J Pathol 177, 415-23. Applications: Western Blotting
• Goto, J. et al. (2011) Proc Natl Acad Sci U S A 108, E1070-9. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP^® Rabbit mAb
• 2217 S6 Ribosomal Protein (5G10) Rabbit mAb
• 1220 Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide
• 2317 S6 Ribosomal Protein (54D2) Mouse mAb
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8101 SignalSlide^® Phospho-Akt (Ser473) IHC Controls
• 8112 SignalStain^® Antibody Diluent

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.