Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

WASP Antibody #4860

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Endogenous 60 human, 62 mouse Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WASP Antibody detects endogenous levels of total WASP protein. The antibody does not cross-react with N-WASP.

Source / Purification

Polyclonal Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human WASP. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using WASP Antibody.

IP

IP

Immunoprecipitation of WASP from Ramos cells, followed by western blot analysis, using WASP Antibody.

Background

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).

  1. Millard, T.H. et al. (2004) Biochem J. 380, 1-17.
  2. Yan, C. et al. (2003) EMBO J. 22, 3602-3612.
  3. Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904.
  4. Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132.
  5. Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302.
  6. Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974.
  7. Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145.
  8. Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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