Product Pathways - Adhesion
Phospho-Zyxin (Ser142/143) Antibody #4863
|4863S||100 µl (10 western blots)||---||In Stock||---|
|4863||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Monkey||Endogenous||78||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-Zyxin (Ser142/143) Antibody recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142, Ser143 or both residues.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser142 of human zyxin protein. Antibodies are purified using protein A and peptide affinity chromatography.
The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1).
Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4).
- Hoffman, L.M. et al. (2012) Mol Biol Cell , . Applications: Western Blotting, IF-IC (In Cells).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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