Product Pathways - Phosphatases
PTP-PEST (AG10) Mouse mAb #4864
|W IP||H M R Mk||Endogenous||110 to 125||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
PTP-PEST (AG10) Mouse mAb detects endogenous levels of total PTP-PEST protein. This antibody does not cross-react with other protein tyrosine phosphatases.
Source / Purification
Monoclonal antibody is produced by immunizing animals with human PTP-PEST recombinant protein. The antibody recognizes an epitope within the amino-terminal 305 residues.
PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASp, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak and WASp, PTP-PEST negatively regulates of lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substates such as paxillin, p130 Cas, Crk and PSTPIP1 (8).
- Davidson, D. and Veillette, A. (2001) EMBO J. 20, 3414-3426.
- Garton, A.J. and Tonks, N.K. (1999) J. Biol. Chem. 274, 3811-3818.
- Shen, Y. et al. (2000) J. Biol. Chem. 275, 1405-1413.
- Angers-Loustau, A. et al. (1999) J. Cell Biol. 144, 1019-1031.
- Cote, J. et al. (2002) J. Biol. Chem. 277, 2973-2986.
- Badour, K. et al. (2003) J. Exp. Med. 199, 99-111.
- Horsch, K. et al. (2001) Mol. Endocrinol. 15, 2182-2196.
- Cong, F. et al. (2000) Mol. Cell 6, 1413-1423.
- Garton, A. J. et al. (1997) Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition. Oncogene 15, 877-885. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.