Cell Signaling Technology

Product Pathways - Transcription Factors

E2A Antibody #4865

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 75 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

E2A Antibody detects endogenous levels of total E2A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu475 of human E2A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using E2A Antibody.

Background

E2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box consensus sequences (1,2). Alternative splicing generates two E2A isoforms (E47 and E12) that are actively involved in B cell lineage commitment, B cell maturation, IgK V-J rearrangement, peripheral B cell development, and tumor suppression (3). E2A acts in cis during G1 to promote immunoglobulin gene diversification (4). Research studies have shown that chromosomal translocations involving the E2A gene result in the expression of multiple fusion proteins and are associated with many cases of pediatric acute lymphoblastic leukemia (5).

  1. Murre, C. (2005) Nat Immunol 6, 1079-86.
  2. Bain, G. et al. (2001) Nat Immunol 2, 165-71.
  3. Schwartz, R. et al. (2006) Proc Natl Acad Sci U S A 103, 9976-81.
  4. Yabuki, M. et al. (2009) J Immunol 182, 408-15.
  5. LeBrun, D.P. (2003) Front Biosci 8, s206-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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