Product Pathways - Cytoskeletal Signaling
VDAC Antibody #4866
PhosphoSitePlus® protein, site, and accession data: VDAC1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R B | Endogenous | 32 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4866:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
VDAC Antibody detects endogenous levels of total VDAC protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human VDAC-1. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using VDAC Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using VDAC Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using VDAC Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using VDAC Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using VDAC Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms, VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5).
- Hodge, T. and Colombini, M. (1997) J Membr Biol 157, 271-9.
- Shimizu, S. et al. (1999) Nature 399, 483-7.
- Zheng, Y. et al. (2004) Oncogene 23, 1239-47.
- Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12.
- Yamamoto, T. et al. (2006) J Proteome Res 5, 3336-44.
Application References
- Qattan, A.T. et al. (2010) J Proteome Res 9, 495-508. Applications: Western Blotting
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Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
For Research Use Only. Not For Use In Diagnostic Procedures.
