Product Pathways - Cytoskeletal Signaling
VDAC Antibody #4866
|4866S||100 µl (10 western blots)||---||In Stock||---|
|4866||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Bovine||Endogenous||32||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
VDAC Antibody detects endogenous levels of total VDAC protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human VDAC-1. Antibodies are purified using protein A and peptide affinity chromatography.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using VDAC Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using VDAC Antibody.
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using VDAC Antibody.
Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms, VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5).
- Qattan, A.T. et al. (2010) J Proteome Res 9, 495-508. Applications: Western Blotting.
- Zhang, J. et al. (2013) Nat Cell Biol , . Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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