Product Pathways - Protein Folding/Stability
HSP60 (D85) Antibody #4869
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H M R Mk Dm | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
HSP60 (D85) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) surrounding Asp85 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using HSP60 (D85) Antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells using HSP60 (D85) Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using HSP60 (D85) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
In both prokaryotic and eukarytic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).
- Hartl, F.U. (1996) Nature 381, 571-579.
- Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
- Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
- Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
- Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
- Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
- Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
- Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
