Product Pathways - Protein Folding
HSP60 (D85) Antibody #4869
|4869S||100 µl (10 western blots)||---||In Stock||---|
|4869||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, D. melanogaster||Endogenous||60||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
HSP60 (D85) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp85 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell types using HSP60 (D85) Antibody.
Flow cytometric analysis of HeLa cells using HSP60 (D85) Antibody (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of HeLa cells using HSP60 (D85) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).
- Hartl, F.U. (1996) Nature 381, 571-579.
- Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
- Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
- Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
- Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
- Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
- Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
- Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.
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