Product Pathways - NF-kB Signaling

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NF-κB2 p100/p52 Antibody #4882

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk	Endogenous	52 (mature). 120 (precursor).	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4882:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

NF-kappaB2 p100 Antibody detects endogenous levels of p100, the precursor, and p52, the mature form of NF-kappaB2. The antibody does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human p100NF-kappaB2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Application References

• He, J.Q. et al. (2006) J Exp Med 203, 2413-8. Applications: Western Blotting
• Solt, L.A. et al. (2007) J Biol Chem 282, 8724-33. Applications: Western Blotting
• Hasler, U. et al. (2008) J Biol Chem 283, 28095-105. Applications: Western Blotting
• Vince, J.E. et al. (2008) J Cell Biol 182, 171-84. Applications: Western Blotting
• Zarnegar, B.J. et al. (2008) Nat Immunol 9, 1371-8. Applications: Western Blotting
• Lu, Y.C. et al. (2009) J Immunol 182, 7212-21. Applications: Western Blotting
• Razani, B. et al. (2010) Sci Signal 3, ra41. Applications: Western Blotting
• Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting
• Lovas, A. et al. (2012) PLoS One 7, e42741. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3017 NF-κB2 p100/p52 (18D10) Rabbit mAb (Human Specific)
• 4810 Phospho-NF-κB2 p100 (Ser866/870) Antibody
• 4954 RelB Antibody
• 2682 IKKα Antibody
• 2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
• 4994 NIK Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.