Product Pathways - Development
TAZ (V386) Antibody #4883
PhosphoSitePlus® protein, site, and accession data: TAZ
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (Hr) | Endogenous | 50 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4883:
- Western Blotting
Specificity / Sensitivity
TAZ (V386) Antibody detects endogenous levels of total TAZ protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues surrounding Val 386 of human TAZ. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using TAZ (V386) Antibody.
Background
TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ appears to be negatively regulated by LATS tumor suppressor kinase, a component of the Hippo pathway that controls tissue growth and tumor development (4).
- Kanai, F. et al. (2000) EMBO J 19, 6778-91.
- Hong, J.H. et al. (2005) Science 309, 1074-8.
- Hong, J.H. and Yaffe, M.B. (2006) Cell Cycle 5, 176-9.
- Lei, Q.Y. et al. (2008) Mol Cell Biol 28, 2426-36.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
