| # | Product Name | Applications | Reactivity | |
|---|---|---|---|---|
| 4806 | Phospho-NF-κB p105 (Ser932) (18E6) Rabbit mAb |
|
H M R Mk |
| REACTIVITY | |
| SENSITIVITY | |
| MW (kDa) | 105 |
| SOURCE | Rabbit |
Product Information
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Ser933 of NF-kappaB p105. Antibodies are purified by protein A and affinity chromatography.
Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
Following IKK-mediated phosphorylation of p105 NF-kappaB at multiple sites (Ser921, 923, 927, and 933) on its carboxy-terminus, SCFbeta-TrCP mediated processing produces the 50kDa active form p50 (12,13)
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