Cell Signaling Technology

Product Pathways - Protein Stability

UBE1a/b Antibody #4891

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Endogenous 110, 117 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

UBE1a/b Antibody detects endogenous levels of total UBE1a and UBE1b proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr800 of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a/b Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-N-MC cells using UBE1a/b Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).

  1. Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
  2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157.
  3. Hochstrasser, M. (2000) Science 289, 563-564.
  4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-189.
  5. Stephen, A.G. et al. (1996) J. Biol. Chem. 271, 15608-15614.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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