Product Pathways - Protein Stability
UBE1a/b Antibody #4891
|4891S||100 µl (10 western blots)||---||In Stock||---|
|4891||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||110, 117||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
UBE1a/b Antibody detects endogenous levels of total UBE1a and UBE1b proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr800 of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a/b Antibody.
Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).
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