Cell Signaling Technology

Product Pathways - DNA Damage

p63-α Antibody #4892

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H (C) Endogenous 75 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  C=Chicken
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

p63alpha Antibody detects endogenous levels of total p63alpha protein. This antibody will detect both full length TAp63alpha and truncated deltaNp63alpha, but not p63beta or p63gamma.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within the carboxy-terminus of p63alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from ME-180 cells, using p63α Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-431 cells (left) and MCF7 cells (right) using p63-α Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40 kDa deltaNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4).

  1. Levine, A. J. et al. (1997) Cell 88, 323-331.
  2. Waltermann, A. et al. (2003) Oncogene 22, 5686-5693.
  3. Hibi, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5462-5467.
  4. Yang, A. et al. (1999) Nature 398, 714-718.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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