Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PLK3 (D14F12) Rabbit mAb #4896

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M (R) (Mk) Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PLK3 (D14F12) Rabbit mAb detects endogenous levels of total PLK3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys625 of human PLK3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human PLK3 construct (+), using PLK3 (D14F12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEL, A549 and A20 cells using PLK3 (D14F12) Rabbit mAb.

Background

At least 4 distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3 and PLK4/SAK (1). Like the other PLK family members, PLK3 contains an amino-terminal catalytic domain and a conserved carboxy-terminal domain termed the Polo box (2). PLK3, also called proliferation-related kinase (Prk) (3), was originally described as a fibroblast growth factor (FGF)-inducible kinase (Fnk) and identified as an immediate-early response gene responsive to FGF-1 and other mitogens (4). PLK3 is a cytokine-inducible serine/threonine kinase whose protein expression is cell cycle regulated. Though its expression is found primarily in G1 phase of the cell cycle, PLK3 is detected in G0 and in late telophase prior to cytokinesis (5). Like the other PLK family members, PLK3 functions mainly as a regulator of the cell cycle. Specifically, PLK3 is required for entry into S phase and is a critical regulator of G1 events, as indicated by RNAi-induced PLK3-depleted cells (2). PLK3 is also involved in the regulation of DNA damage response via phosphorylation of p53 on Ser20 (6). PLK3 may act as a tumor suppressor as Plk3-deficient mice develop spontaneous tumors in various organs (7). Unlike PLK1, PLK3 expression is down regulated in cancers including lung (3), head and neck (8), and colon (9).

  1. Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83.
  2. Zimmerman, W.C. and Erikson, R.L. (2007) Proc Natl Acad Sci USA 104, 1847-52.
  3. Li, B. et al. (1996) J Biol Chem 271, 19402-8.
  4. Donohue, P.J. et al. (1995) J Biol Chem 270, 10351-7.
  5. Zimmerman, W.C. and Erikson, R.L. (2007) Cell Cycle 6, 1314-8.
  6. Xie, S. et al. (2001) J Biol Chem 276, 43305-12.
  7. Yang, Y. et al. (2008) Cancer Res 68, 4077-85.
  8. Dai, W. et al. (2000) Genes Chromosomes Cancer 27, 332-6.
  9. Dai, W. et al. (2002) Int J Oncol 20, 121-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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