Product Pathways - Development
Sox2 (L1D6A2) Mouse mAb #4900
|W IF-IC F||H M (R) (B) (Dg) (Hr)||Endogenous||35||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine Dg=Dog Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Sox2 (L1D6A2) Mouse mAb detects endogenous levels of total Sox2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein.
Western blot analysis of extracts from NTERA-2, F9 and P16 cells using Sox2 (L1D6A2) Mouse mAb.
Flow cytometric analysis of HeLa (red) and NTERA-2 cells (blue) using Sox2 (L1D6A2) Mouse mAb.
Confocal immunofluorescent analysis of NTERA-2 (left), HeLa (center) and mouse ES cells growing on MEF feeder cells (right) using Sox2 (L1D6A2) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
- Nichols, J. et al. (1998) Cell 95, 379-391.
- Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
- Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
- Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
- Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
- Boyer, L.A. et al. (2005) Cell 122, 947-956.
- Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
- Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.
- Zhu, X. et al. (2013) Cancer Res 73, 1142-55. Applications: Western Blotting
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3728 Sox2 (C70B1) Rabbit mAb (IHC Preferred)
- 3579 Sox2 (D6D9) XP® Rabbit mAb
- 2840 Oct-4A (C30A3) Rabbit mAb
- 2890 Oct-4A (C52G3) Rabbit mAb
- 3580 Nanog Antibody
- 4903 Nanog (D73G4) XP® Rabbit mAb
- 9656 StemLight™ Pluripotency Antibody Kit
For Research Use Only. Not For Use In Diagnostic Procedures.