Cell Signaling Technology

Product Pathways - Development

Sox2 (L1D6A2) Mouse mAb #4900

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H M (R) (B) (Dg) (Hr) Endogenous 35 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine  Dg=Dog  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Sox2 (L1D6A2) Mouse mAb detects endogenous levels of total Sox2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA-2, F9 and P16 cells using Sox2 (L1D6A2) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (red) and NTERA-2 cells (blue) using Sox2 (L1D6A2) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 (left), HeLa (center) and mouse ES cells growing on MEF feeder cells (right) using Sox2 (L1D6A2) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).


Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

  1. Nichols, J. et al. (1998) Cell 95, 379-391.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
  3. Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
  4. Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
  5. Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
  6. Boyer, L.A. et al. (2005) Cell 122, 947-956.
  7. Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
  8. Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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