Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PI4 Kinase Antibody #4902

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M R Endogenous 230 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PI4 Kinase Antibody detects endogenous levels of total PI4 Kinase isoform 230.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of PI4 Kinase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1, MDA-MB-468 and CAD cells, using PI4 Kinase Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using PI4 Kinase Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters. Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP). In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD. Previously, PI4 kinases were classified into type II and III enzymes (2). All isoforms are located on distinct membranes and cellular compartments suggesting various tasks. PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes (3-5). PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives (3, 6).

  1. Wong, K. and Cantley, L.C. (1994) J. Biol. Chem. 269, 28878-28884.
  2. Heilmeyer Jr., L. M. et al. (2003) IUBMB Life. 55, 59-65.
  3. Balla, A. et al. (2000) Exp. Brain Res. 134, 279-288.
  4. Nakagawa, T. et al. (1996) Biochem. J. 320, 643-649.
  5. Balla, A. et al. (2002) J. Biol. Chem. 277, 20041-20050.
  6. Pelyvas, I. F. et al. (2001) J. Med. Chem. 44, 627-632.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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