Product Pathways - Jak/Stat Pathway
Stat3 (79D7) Rabbit mAb #4904
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R Mk | 79, 86 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Stat3 (79D7) Rabbit mAb detects endogenous levels of total Stat3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with GST-Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3.
Western Blotting
Western blot analysis of extracts from NIH/3T3, COS, PC12 and SK-N-MC cells, using Stat3 (79D7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Stat3 (79D7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear and cytoplasmic localization, using Stat3 (79D7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Stat3 (79D7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Stat3 (79D7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma, using Stat3 (79D7) Rabbit mAb.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120.
- Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801-3804.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-115.
- Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell Jr., J.E. et al. (1994) Science 264, 1415-1421.
- Ihle, J.N. (1995) Nature 377, 591-594.
- Wen, Z. et al. (1995) Cell 82, 241-250.
- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9132 Stat3 Antibody
- 9133 Stat3 Control Cell Extracts
- 9134 Phospho-Stat3 (Ser727) Antibody
- 9136 Phospho-Stat3 (Ser727) (6E4) Mouse mAb
- 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
- 9139 Stat3 (124H6) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9145 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb
- 8105 SignalSlide™ Phospho-Stat1/3/5 IHC Controls
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
