Cell Signaling Technology

Product Pathways - Chromatin Regulation

Ezh2 Antibody #4905

Applications Reactivity MW (kDa) Source
W IP H M R Pg 98 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Ezh2 Antibody detects endogenous levels of Ezh2 protein. The antibody may cross-react with Ezh2-related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the amino-terminus of human Ezh2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ezh2 Antibody.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

  1. Seller, W.B. and Loda, M. (2002) Cancer Cell 2, 349-350.
  2. Visser, H.P. et al. (2001) Br. J. Haematol. 112, 950-958.
  3. Chen, H. et al. (1996) Genomics 38, 30-37.
  4. Tonini, T. et al. (2004) Oncogene 23, 4930-4937.
  5. Muller, J. et al. (2002) Cell 111, 197-208.
  6. Kleer, C.G. et al. (2003) Proc Natl. Acad. Sci. USA 100, 11606-11611.

Application References

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