Product Pathways - PI3K / Akt Signaling
YAP Antibody #4912
PhosphoSitePlus® protein, site, and accession data: YAP1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk | Endogenous | 65 to 75 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
YAP Antibody detects endogenous levels of total YAP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3, C6 and MDA-MB-231 cells, using YAP Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using YAP Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using YAP Antibody in the presence of control peptide (left) or antigen specific peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic and nuclear localization, using YAP Antibody.
Flow Cytometry
Flow cytometric analysis of untreated C6 cells, using YAP antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells (left) and Jurkat cells (right) using YAP Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).
Background
YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6).
- Sudol, M. (1994) Oncogene 9, 2145-2152.
- Mohler, P. et al. (1999) J. Cell Biol. 147, 879-890.
- Espanel, X. and Sudol, M. (2001) J. Biol. Chem. 276, 14514-14523.
- Sudol, M. et al. (1995) FEBS Lett. 369, 67-71.
- Yagi, R. et al. (1999) EMBO J. 18, 2551-2562.
- Basu, S. et al. (2003) Mol. Cell 11, 11-23.
Application References
- Jellicoe, M.M. et al. (2008) Carcinogenesis 29, 1124-33. Applications: Western Blotting
- Poon, C.L. et al. (2011) Dev Cell 21, 896-906. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4911 Phospho-YAP (Ser127) Antibody
- 4691 Akt (pan) (C67E7) Rabbit mAb
- 9271 Phospho-Akt (Ser473) Antibody
- 9275 Phospho-Akt (Thr308) Antibody
- 9935 Src Antibody Sampler Kit
- 4662 p73 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8112 SignalStain® Antibody Diluent
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
