Cell Signaling Technology

Product Pathways - Adhesion

Hic-5 Antibody #4914

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk B Endogenous 50 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Hic-5 Antibody detects endogenous levels of total Hic-5/ARA55 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Ala104 of human Hic-5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U-2-OS, COS and A431 cells using Hic-5 Antibody.

IP

IP

Immunoprecipitation of Hic-5 from 293 cell lysates using Hic-5 Antibody or rabbit IgG control. Western blot was performed using a Hic-5 Mouse mAb.

Background

Hic-5 is a LIM domain family member orginally identified as a TGFbeta1 and hydrogen peroxide inducible gene, and is nearly identical to the androgen receptor co-activator ARA55 (1-3). Hic-5 is structurally related to paxillin, and both proteins are localized to focal adhesions and thought to serve as adaptor molecules, linking signals from the extracellular matrix to cytoskeletal regulation and intracelluar signaling (4,5). Like paxillin, Hic-5 contains four LD motifs and four LIM domains. Expression of Hic-5 can affect cell growth and differentiation (6-8). Increased expression of Hic-5 is observed during cellular senescence in fibroblasts, and ectopic expression in immortalized fibroblasts suppressed cell growth (8). Unlike paxillin, Hic-5 may translocate to the nucleus in response to oxidants like hydrogen peroxide (9). It has been proposed that Hic-5 serves to shuttle redox signaling from focal adhesions to the nucleus where it acts as a transcriptional co-activator for some transciption factors including, Sp1 and PPARgamma (7,9,10). Phosphorylation of Hic-5 at Tyr60 by CAKbeta and Fyn may activiate Hic-5 signaling by allowing binding to downstream SH2 domain containing proteins (11).

  1. Ohba, M. et al. (1994) J. Cell Biol. 126, 1079-1088.
  2. Shibanuma, M. et al. (1994) J. Biol. Chem. 269, 26767-26774.
  3. Fujimoto, N. et al. (1999) J. Biol. Chem. 274, 8316-8321.
  4. Matsuya, M. et al. (1998) J. Biol. Chem. 273, 1003-1014.
  5. Nishiya, N. et al. (2001) Mol. Cell. Biol. 21, 5332-5345.
  6. Hu, Y. et al. (1999) Proc. Natl. Acad. Sci. 96, 10218-10223.
  7. Drori, S. et al. (2005) Genes Dev. 19, 362-375.
  8. Shibanuma, M. et al. (1997) Mol. Cell. Biol. 17, 1224-1235.
  9. Shibanuma, M. et al. (2003) Mol. Biol. Cell 14, 1158-1171.
  10. Shibanuma, M. et al. (2004) J. Cell. Biochem. 91, 633-645.
  11. Ishino, M. et al. (2000) FEBS Lett. 474, 179-183.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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