Cell Signaling Technology

Product Pathways - Adhesion

CD54 (ICAM-1) Antibody #4915

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P F H Endogenous 89, 92 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

ICAM-1 Antibody detects endogenous levels of total ICAM-1 protein. The antibody does not cross-react with other IgSF adhesion molecules.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues of human ICAM-1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos, A549, or Raji cells, treated with TNF-alpha as indicated, using ICAM-1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymph node, using ICAM-1 Antibody in the presence of control peptide (left) or antigen specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Raji cells, using ICAM-1 Antibody.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Raji cells, using ICAM-1 antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Intercellular cell adhesion molecule-1 (ICAM-1), also known as CD54, is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. ICAM-1 is expressed at low levels on diverse cell types, and is induced by cytokines (TNFalpha, interleukine-1) and bacterial lipopolysaccharide (1). Apical localization of ICAM-1 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier (1). Apical expression of ICAM-1 on epithelial cells mediates pathogen invasion as well as host defense, and this pattern is also observed in tumors (1). ICAM-1 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 ((leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation (2). Cross-linking of ICAM-1 or binding to its ligands triggers activation of Src family kinases as well the Rho/ROCK pathway (3-7). Phosphorylation on Tyr485 of ICAM-1 is required for its association with SHP-2 (5). SHP-2 seems essential for ICAM-1-induced Src activation (7).

  1. Hopkins, A. M. et al. (2004) Adv. Drug Deliv. Rev. 56, 763-778.
  2. Nishibori, M. et al. (2003) J. Pharmacol. Sci. 92, 7-12.
  3. Holland, J. and Owens, T. (1997) J. Biol. Chem. 272, 9108-9112.
  4. Etienne, S. et al. (1998) J. Immunol. 161, 5755-5761.
  5. Pluskota, E. et al. (2000) J. Biol. Chem. 275, 30029-30036.
  6. Muro, S. et al. (2003) J. Cell Sci. 116, 1599-1609.
  7. Wang, Q. et al. (2004) J. Biol. Chem. 278, 47731-47743.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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