Cell Signaling Technology

Product Pathways - Adhesion

CD54 (ICAM-1) Antibody #4915

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H Endogenous 89, 92 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD54 (ICAM-1) Antibody detects endogenous levels of total CD54 protein. The antibody does not cross-react with other IgSF adhesion molecules.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human CD54. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos, A549 and Raji cells, untreated or TNF-α-treated, using CD54 (ICAM-1) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymph node using CD54 (ICAM-1) Antibody in the presence of control peptide (left) or antigen specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Raji cells using CD54 (ICAM-1) Antibody.


Background

Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide (1). Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier (1). Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors (1). CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation (2). Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway (3-7). Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2 (5). SHP-2 seems essential for CD54-induced Src activation (7).

  1. Hopkins, A. M. et al. (2004) Adv. Drug Deliv. Rev. 56, 763-778.
  2. Nishibori, M. et al. (2003) J. Pharmacol. Sci. 92, 7-12.
  3. Holland, J. and Owens, T. (1997) J. Biol. Chem. 272, 9108-9112.
  4. Etienne, S. et al. (1998) J. Immunol. 161, 5755-5761.
  5. Pluskota, E. et al. (2000) J. Biol. Chem. 275, 30029-30036.
  6. Muro, S. et al. (2003) J. Cell Sci. 116, 1599-1609.
  7. Wang, Q. et al. (2004) J. Biol. Chem. 278, 47731-47743.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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