Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

STIM1 Antibody #4916

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 80 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

STIM1 Antibody detects endogenous levels of total STIM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human STIM1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, PC12 and COS cells using STIM1 Antibody.

Background

Ca2+ is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca2+ sensors that detect changes in Ca2+ content in intracellular Ca2+ stores (3). STIM1 is conserved and ubiquitously expressed and functions as an endoplasmic reticulum Ca2+ sensor that that migrates from the endoplasmic Ca2+ store to the plasma membrane to activate calcium-release-activated calcium (CRAC) channels when the endoplasmic Ca2+ store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca2+ entry (6), however its true physiological function requires further investigation.

  1. Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21.
  2. Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29.
  3. Zheng, L. et al. (2008) Biochem Biophys Res Commun 369, 240-6.
  4. Zhang, S.L. et al. (2005) Nature 437, 902-5.
  5. Manji, S.S. et al. (2000) Biochim Biophys Acta 1481, 147-55.
  6. Soboloff, J. et al. (2006) Curr Biol 16, 1465-70.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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