Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

STIM1 Antibody #4916

Applications Reactivity MW (kDa) Source
W IP H M R Mk 80 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

STIM1 Antibody detects endogenous levels of total STIM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human STIM1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, PC12 and COS cells using STIM1 Antibody.

Background

Ca2+ is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short term responses such as contraction and secretion to longer term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca2+ sensors to detect changes in Ca2+ content in intracellular Ca2+ stores (3). STIM1 is conserved and ubiquitously expressed and functions as an endoplasmic reticulum Ca2+ sensor that that migrates from the endoplasmic Ca2+ store to the plasma membrane to activate calcium-release-activated calcium (CRAC) channels when the endoplasmic Ca2+ store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca2+ entry (6), however its true physiological function still requires further investigation.

  1. Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21.
  2. Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29.
  3. Zheng, L. et al. (2007) Biochem Biophys Res Commun, Epub ahead of print.
  4. Zhang, S.L. et al. (2005) Nature 437, 902-5.
  5. Manji, S.S. et al. (2000) Biochim Biophys Acta 1481, 147-55.
  6. Soboloff, J. et al. (2006) Curr Biol 16, 1465-70.

Application References

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